Rice seedling disease is an important disease on rice and a seed-borne disease caused by fungi. The benzoxazole drug represented by carbendazim was once the main agent for the prevention and treatment of the disease. With the resistance of the pathogen to the carbendazim, the single use of the drug decreased. Prochloraz is an imidazole fungicide. In recent years, it has achieved good control effect against rice seedling disease, but at present some local areas have also experienced a decline in control effect.
There are three main reasons for the occurrence of rice seedling disease: one is that the rice is not treated before seeding; the other is that the seed treatment agent is not properly selected; the third is that the use of technology is not in place. In view of this situation, three points should be done in prevention and control: First, comprehensive seed treatment before rice planting, killing germs on the seeds, requiring seed treatment to reach 100%, if missing, it may cause disease; second, correct selecting a seed treatment, the current control of rice Bakanae preferably biocides prochloraz; Third master the correct use of technology. The key points are two points: First, the concentration of use: depending on the pesticide label and instructions, the concentration of different pesticides is different, such as 25% of the 25% prochloraz hybrid rice seeds and 4000 times of the conventional rice seeds; the second is the soaking time: soaking seeds The time is related to the choice of the agent and the temperature. For example, the time for soaking the prochloraz can be shorter, the soaking time with high temperature is short, and the temperature is low for soaking. Generally, the early rice is soaked in 2 to 3 days (48 to 72 hours), and the late rice is soaked in 1 to 2 days (24 to 48 hours).
Regarding the resistance of prochloraz, the researchers of the College of Agronomy and Biotechnology of China Agricultural University and the Institute of Pesticide Testing of the Ministry of Agriculture used mycelial growth rate and microscopic observation to study the sensitive strains and anti-resistant strains of prochloraz on rice seedling pathogens. The effects of growth and development and mycelial growth morphology of sexual mutant strains. The results showed that: 0.5 mg / liter of prochloraz solution can completely inhibit the mycelial growth of sensitive strains, can not inhibit the mycelial growth of resistant strains with different resistance levels; 20 mg / liter of prochloraz solution can completely inhibit the parents The spore germination of sensitive strains inhibited the spore germination and germ tube elongation of different resistant mutants by 84.1%-89% and 58.5%-65.8%, respectively, indicating that the resistant mutant strains grew in mycelium, spore germination, The sensitivity to the agent at the stage of elongation of the germ tube is reduced to a different extent than that of the parental sensitive strain. After treating the hyphae with 10 mg/L of prochloraz solution for 12 hours, the hyphae of the parental sensitive strain expanded and deformed, and the hyphal top of the resistant mutant expanded, but there were many small branches on the hyphae, indicating that the agent was antagonistic. The inhibitory effect of the secondary mutant hyphae of the mutant was not obvious.
The above test results indicate that the rice seedling pathogen has potential drug resistance risk to prochloraz, and it is recommended to rotate the drug with a different mechanism of action such as dithiocyanomethane or trichloroisocyanuric acid to delay or Avoid drug resistance.
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