Beijing Xiao Li Q: How to make Pleurotus ostreatus, how to inoculate?
Chen Wenliang Researcher Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences
A: The production method of Pleurotus ostreatus is as follows:
â– Prepare the wood strip and soak it in water for 3-5 hours.
■Put the wooden strip into the strain bottle, and after autoclaving for 2-3 hours, or atmospheric pressure sterilization (at 100 °C) for more than 12 hours, completely eliminate the bacteria in the wood strip.
â– Access the Pleurotus ostreatus to the wood strip bundle under sterile conditions in the inoculation room.
■Under the culture conditions of 25 ° C, the wood spores grow well after 25-30 days.
Inoculation method using wood stalks:
â– Insert a sterile hole of about 10 cm into the sterilized bag with the sterilized stainless steel strip, and then insert the sclerotium into the hole, and insert 3-4 strains per bag. Upper plastic collar or nylon cord seal.
■Culture temperature 22 ° C -25 ° C.
â– When inoculation, it should be carried out under aseptic conditions. Generally, Biejieshi chlorine dioxide disinfectant fumigation disinfection chamber (box) is used.
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